| Address: | 6221 Corona Ave, Bell, CA 90201, USA |
|---|---|
| Postal code: | 90201 |
| Phone: | (323) 773-0248 |
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To better understand the maximum imaging depths that we can achieve in epithelial tissues, we studied The phantom data agree well with the estimated contrast decays from time-resolved Monte Carlo simulations and show maximum imaging depths similar to that found in human biopsy results.
Durr et al.: Maximum imaging depth of two-photon autofluorescence microscopy... Out-of-focus (background) fluorescence gradually reduces the image contrast.18, 19 The ratio of collected signal fluorescence (Fs ) to background fluorescence (Fb ) then decays with increasing imaging depth.
To better understand the maximum imaging depths that we can achieve in epithelial tissues, we studied The phantom data agree well with the estimated contrast decays from time-resolved Monte Carlo simulations and show maximum imaging depths similar to that found in human biopsy results.
The maximum imaging depth can also be improved when the sample is illuminated from multiple directions, as demonstrated by double-illumination PAM (DI-PAM) [57]. [50] K. Passler, R. Nuster, S. Gratt, P. Burgholzer, T. Berer, and G. Paltauf, Biomedical Optics Express 1(1), 318-323 (2010).
at the maximum imaging depth of 420μm. The paper demonstrates that THG imaging using 1552 nm as an illumination wavelength with effective thermal management proves to be a powerful deep imaging modality for highly scattering and absorbing tissues, such as scarred vocal folds.
The maximum imaging depth zmax may be doubled by the use of methods for removing the complex conjugate ambiguity artifact. The maximum probing depth is of considerable interest in the characterization and optimization of an OCT system when used for imaging in highly scattering tissue.
To better understand the maximum imaging depths that we can achieve in epithelial tissues, we studied image contrast as a function of depth in tissue phantoms with a range of relevant optical properties. image contrast. 8. imaging depth. 8. epithelial. 5. imaging. 5. maximum. 4. reached 160.
Ben-Yakar Lab: FemtoLab: Femtosecond Laser Nanosurgery, Two Photon Imaging, Plasmonic Surgery and Imaging, Micromachining, Microfluidics. Durr, N.J., C. T. Weisspfennig, B. A. Holfeld, A. Ben-Yakar, "Maximum imaging depth of two-photon autofluorescence microscopy in epithelial...
To better understand the maximum imaging depths that we can achieve in epithelial tissues, we studied image contrast as a function of depth in tissue phantoms with a range of relevant optical properties. The phantom data agree well with the estimated contrast decays from time-resolved Monte...